This application shows the main features and work-flow of the IVIS Optical Imaging platform from Caliper Life Sciences. It is meant to be a helper tool for
in-vivo bioluminescent and fluorescent imaging work-flow on an IVIS using the Living Image software.
Some of the imaging and analysis options portrayed are Fluorescence Spectral Unmixing, DLIT (Diffuse Luminescence Imaging Tomography) and FLIT (Fluorescent
Imaging Tomography) based 3D reconstructions, Kinetic Imaging and Lumina XR capabilities.
This application comes with a very useful filter selection tool to help IVIS users determine optimum selection of Emission and Excitation filters for a
selected IVIS system and imaging options.
IVIS Optical Imaging Platform Features:
- Bioluminescence 2D in-vivo imaging
- Fluorescence 2D in-vivo imaging
- Fluorescence Spectral Unmixing in-vivo imaging
- Fluorescence Spectra for fluorescent tags and proteins
- 2D bioluminescence and 3D (DLIT) in-vivo imaging
- Epi & Trans fluorescence 3D (FLIT) in-vivo imaging
- Overlay of 3D bioluminescent and fluorescent sources
- X-Ray in-vivo imaging co-registered with bioluminescent/fluorescent images
- Real-Time Kinetic in-vivo imaging co-registered with bioluminescent/fluorescent images
Filter Selection Tool
The Filter Selection Tool is an integral part in guiding IVIS users in their in-vivo bioluminescent and fluorescent imaging protocol.
It offers a simple interface to select an optical reporter and/or probe with an optimal set of Emission and Excitation filters for in-vivo applications. This
is illustrated by a bar graph representing available filters on the system and a spectrum plot representing the selected probe, with the recommended filters
highlighted.
- Emission and Excitation Filter Selection Tool for IVIS Optical Imaging
- Bioluminescent and Fluorescent probe selections
- Visual bar chart based Emission & Excitation filter selection recommendations